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Co-immunostaining using antibodies against glutamine synthetase, glial fibrillary acidic protein, choline acetyltransferase, and CD31 was applied to identify specific cell types. In the adult eye, a persistent and very strong promotor activity was present in the trabecular meshwork outflow pathways. Very strong promoter activity was seen in the astrocytes of the glial lamina at the optic nerve head.
CCN2 is a member of the cellular communication network CCN family of extracellular matrix-associated and heparin-binding matricellular proteins Brigstock ; Leask ; Perbal and Perbal ; Perbal , The increase in trabecular meshwork contractility causes dysfunction of the aqueous humor outflow pathways, high intraocular pressure and glaucoma Junglas et al.
Immunohistochemical studies are available that studied its localization in ocular tissues Pi et al. Feeding was ad libitum. Before enucleation of the eyes, mice were anesthetized with CO 2 and euthanized by atlanto-occipital dislocation. RNA that was not reverse transcribed served as negative control. For relative quantification of the experiments, RPL32 was used as a housekeeping gene.
Western blot analysis was performed with specific antibodies as described previously Fuchshofer et al. For light and transmission electron microscopy, eyes were obtained from young adult animals at 9 weeks of age.
